DNA Technology and Genetics

1) What is biotechnology?

The manipulation of organisms or their components to make useful products.

2) List some methods of biotechnology!

1.Bacteria Transformation

2.Restriction Enzymes

3.cDNA

4.DNA Sequencing

5.PCR

6.RFLP

3) What are the steps for plasmid use?

1. Get the DNA for the trait.

2. Insert DNA into the plasmid.

3. Bacterial Transformation.

4. Identification of the new trait.

Facts:

- The practical applications of DNA technology affect our lives in many ways.

- DNA cloning and other techniques, collectively termed DNA technology, can be used to manipulate

and analyze DNA and to produce useful new products and organisms.

- Restrictive enzymes cut DNA at specific nucleotide sequences called “restriction sites”

- Markers are inherited in a Mendelian pattern and can show relationships (Pedigree studies).

- PCR can produce many copies of a specific target segment of DNA, using primers that bracket the

desired sequence and a heat-resistant DNA polymerase.

Summary:

DNA cloning yields multiple copies of a gene or other DNA segment. DNA technology allows us to

study the sequence, expression, and function of a gene. The DNA practical applications of DNA

technology affect our lives in many ways.

Extra:

The PCR song